Sciatic nerve-conditioned medium with the addition of Croton blanchetianus Baill essential oil promotes morphological plasticity in spinal cord cultured cells

Spinal cord injury is a devastating condition that leads to significant disabilities. The treatment for this problem challenge in neuroscience, and it necessary combine different strategies obtain functional recovery outcomes. There are many herbal natural products, such as Croton blanchetianus Baill (CB) essential oil, Brazilian semi-arid bush with neuroprotective substances tested regeneration processes synaptic plasticity. Thus, study analyzed the cellular plasticity of spinal neurons glial cells presence sciatic nerve-conditioned medium (SNCM) before addition CB oil. Cell morphology was assessed over 96 hours, immunocytochemistry analyses were conducted GFAP, GAP-43 NeuN. Photomicrographs made by scanning electron microscopy (SEM). Morphological analysis showed evident trophic development groups received oil (P>0.000), immunoreactivity NeuN these confirmed SEM. This pioneer about opens new possibilities techniques oils cell therapy SNCM, which promoted action.